8 Best Free Online DNA Concentration Calculator Websites

Here is a list of best free online DNA concentration calculator websites. DNA concentration is the amount of DNA present in a given sample. It is usually measured in micrograms per milliliter (µg/mL) unit. It is important to measure the DNA concentration as it helps genetics researchers find out the amount of DNA available for applications like PCR, DNA Sequencing, Cloning, etc. To calculate the concentration of DNA in a given sample, you can try these DNA concentration calculators.

These websites allow users to find out the exact concentration of DNA in a given sample. Most of these websites can work with single-stranded DNA, double-stranded DNA, and RNA samples. Besides this, they also need conversion factor, absorbance value, and dilution factor values to perform the calculation. The process to calculate. Some of these websites, also explain the calculation process and show the calculation formula that they use. To help out new users, I have also included the steps of calculation in the description of each website.

These websites also come with additional online tools like RNA Calculator, Molecular Weight Calculator, Regression Graphing Calculator, etc. Go through the list to know more about these websites.

My Favorite Online DNA Concentration Calculator Website:

aatbio.com is my favorite website as it supports both single-stranded and double-stranded DNAs. Plus, it shows the calculation formula and basic calculation steps.

You can also check out lists of best free Online Thermal Expansion, Golden Ratio Calculator, and Dipole Antenna Calculator websites.

Comparison Table:

Features/Website Names	Supports both single staranded and double staranded DNAs	Shows Calculation Formula	Provides steps of calculation
aatbio.com	✓	✓	✓
omnicalculator.com	✓	✓	x
calctool.org	✓	✓	x
molbiotools.com	x (only supports double stranded DNA)	x	x
nebiocalculator.neb.com	✓	✓	x
calconcalculator.com	✓	x	x
endmemo.com	✓	✓	x
toponlinetool.com	✓	x	x

aatbio.com

aatbio.com is a free online DNA concentration calculator website. Using this website, users can find out the concentration or quantity of DNA in a given sample. Plus, it supports both Single Strand DNA and Double Strand DNA as input. Besides this, users can also enter a custom DNA sequence. Apart from DNA type, it also needs Absorbance (λmax), dilution factor, and path length values to perform the calculation.

This website also shows the calculation formula and basic calculation steps to perform DNA concentration calculation. Now, follow the below steps.

How to perform DNA concentration calculation online using aatbio.com:

Go to this website and select the DNA Concentration Calculator.
After that, specify the DNA type (ssDNA, dsDNA, or Custom Sequence).
Next, enter Absorbance, dilution factor, and pathlength values,
Finally, click on the Calculate button to view the DNA concentration in micrograms per milliliters.

Additional Features:

This website also comes with additional tools like Protein Calculator, RNA Calculator, Molecular Weight Calculator, Regression Graphing Calculator, and more.

Final Thoughts:

It is a capable online DNA concentration calculator website that helps users calculate DNA concentration in a given sample.

Pros	Cons
Shows calculation formula and calculation steps

Home Page

omnicalculator.com

omnicalculator.com is another free online DNA concentration calculator website. This calculator can work with Single-Stranded DNA, Double-Stranded DNA, RNA, and Custom Oligonucleotide samples. Apart from sample type, it also needs conversion factor, path length, and dilution factor values to find out the concentrations of DNA.

This website also shows steps of calculation and formulas that it used to find out the concentration of DNA in a sample. Now, follow the below steps.

How to perform DNA concentration calculation online using omnicalculator.com:

Go to this website using the given link.
After that, select a sample type from the available ones.
Next, enter the conversion factor, absorbance, path length, and dilution factor values.
Finally, view the calculated concentration values in µg/mL unit.

Additional Features;

On this website, users can find multiple online calculators covering various fields like Physics, Maths, Chemistry, Engineering, Thermodynamics, and more.

Final Thoughts:

It is another good online DNA concentration calculator that also explains the steps to calculate DNA concentration.

Pros	Cons
Shows calculation formula
Explains the calculation process

Home Page

calctool.org

calctool.org is another free online DNA concentration calculator website. Through this website, users can calculate DNA concentration in a given sample. Plus, it also explains the process to calculate DNA concentration from the absorbance. Besides this, it can also be used to calculate the concentration of any nucleotide sequence. All essential calculation formulas are also shown by it.

To perform the calculation, users need to specify the Sample Type (single-stranded DNS, double-stranded DNA, etc.), conversion factor, Asorbancem Pathlength, and Dilution Factor, values. Now, follow the below steps.

How to perform DNA concentration calculation online using calctool.org:

Visit this website using the given link.
After that, select a sample type and enter the conversion factor.
Next, enter other required values like Absorbance, Pathlength, and Dilution Factor.
Finally, view the calculated DNA concentration in µg/mL.

Additional Features;

This website also comes with additional calculators associated with Dynamics, Electrical Energy, Electronics, Kinametics, etc., fields.

Final Thoughts:

It is another simple online DNA concentration calculator website that offers all the tools to calculate DNA concentration in a given sample.

Pros	Cons
Explains calculation process
Shows calculation formulas

Home Page

molbiotools.com

molbiotools.com is another free online DNA concentration calculator website. This website offers a DNA calculator that can calculate various physical and chemical parameters including the concentration of DNA in a sample. However, it DNA concentration calculator only supports double-stranded DNA. Besides DNA type, users also need to enter absorbance and dilution factor values to calculate the original DNA concentration value. Unlike other similar websites, it lacks the DNA concentration calculation steps and formulas.

How to perform DNA concentration calculation online using molbiotools.com:

Start this website using the given link.
After that, choose Absorbance and Concentration calculator.
Next, enter the required input values.
Finally, click on the Calculate button to view the DNA concentration value.

Additional Features:

This website also offers additional DNA calculators such as Molarity, Mass, and Volume Calculators.

Final Thoughts:

It is another capable online DNA concentration calculator that can calculate both DNA concentration and absorbance values.

Pros	Cons
	Lacks calculation steps and formulas

Home Page

nebiocalculator.neb.com

nebiocalculator.neb.com is another free online DNA concentration calculator website. This website mainly offers a Nucleic Acids: OD260 Converter that can also be used to calculate the DNA concentration calculation. This calculator needs sample OD or Sample Optical Density value and DNA sample type (double-stranded DNA, single-stranded DNA, single-stranded RNA, etc.) to perform the calculation. It also shows the formula that it uses to find out the DNA concentration in a Sample OD at 260nm. Now, follow the below steps.

How to perform DNA concentration calculation online using nebiocalculator.neb.com:

Go to this website and access the Nucleic Acida: OD260 Converter.
After that, enter the Sample OD value and specify the sample type.
Finally, view the calculated concentration values in microgram per millimeter unit.

Additional Features:

This website also comes with additional online tools like Mass to Moles Converter, Moles to Mass Converter, Dilution Calculator, Molarity Calculator, etc.

Final Thoughts:

It is another good website to perform online DNA concentration calculations.

Pros	Cons
It shows the calculation formulas and explains all parameters

Home Page

calconcalculator.com

calconcalculator.com is another free online DNA concentration calculator website. This website comes with a dedicated DNA Concentration Calculator that works with single-stranded DNA, double-stranded DNA, RNA, and Customized Oligonucleotide. Besides this, it requires conversion factor, absorbance, path length, dilution factor, and concentration values as input. This website also answers common questions related to DNA concentration calculation. Although, it doesn’t explain the process of calculation, unlike some other similar websites. Now, follow the below steps.

How to perform DNA concentration calculation online using calconcalculator.com:

Go to this website and open up the DNA Concentration calculator.
After that, choose a sample type from four available ones.
Next, enter all other required values.
Finally, view the calculated concentration value.

Additional Features:

On this website, users can also find additional online calculators such as Sod, Bird Age, Dog Water Intake, Plant Population, etc.

Final Thoughts:

It is another simple online DNA concentration calculator that helps users calculate DNA concentration of different types of input samples.

Pros	Cons
Answers common questions related to DNA concentration	Lacks calculation formula

Home Page

endmemo.com

endmemo.com is another free online DNA concentration calculator website. Using this website, users can calculate DNA OD260 Nucleotide concentration. Besides this, it can also calculate the concentration of nucleic acid RNA, ssDNAm, and ssOligo. It also shows the formula that it uses to calculate the absorbance of light and nucleic acid concentration. Now, follow the below steps.

How to calculate nucleic-acid DNA concentration online using endmemo.com:

Go to this website using the given link.
After that, enter the D260 value.
Next, select the DNA option.
Finally, click on the Calculate button to get the DNA concentration value in µg/mL.

Additional Features:

This website also comes with calculators and conversion tools like Unit Conversion, Physics Calculator, Algebra Calculator, Medical Calculator, and more.

Final Thoughts:

It is another simple online DNA concentration calculator website that helps users calculate nucleic-acid DNA concentration for a given D260 value.

Pros	Cons
Shows calculation formula	Lacks calculation formula

Home Page

toponlinetool.com

toponlinetool.com is the last free online DNA concentration calculator website. This website offers a simple DNA concentration calculator that works with single-stranded DNA, double-stranded DNA, RNA, and custom oligonucleotide. Before starting the calculation process, users need to specify conversion factor, absorbance, path length, and dilution factor values. After the calculation, it shows the DNA concentration value in micrograms, milligrams, and nanograms per millimeter. Now, follow the below steps.

How to calculate nucleic-acid DNA concentration online using toponlinetool.com:

Go to this website using the given link.
After that, specify the Product type from the available options.
Next, enter other required values.
Finally, click on the Calculate button to view the DNA concentration value.

Additional Features:

On this website, users also get some additional calculators such as boiling point at altitude, bolt circle, carpet calculator, decking calculator, dilution factor, rolling offset, and more.

Final Thoughts:

It is another simple and effective online DNA concentration calculator that anyone can use with ease.

Pros	Cons
	Lacks calculation formula and calculation steps

Home Page

Frequently Asked Questions

What is normal DNA concentration?

In Genomic DNA, the concentration of DNA cab range from a few ng/µL to several hundred ng/µL, with higher concentrations often found in more concentrated DNA preparations or specific cell types. On the other hand, the concentration of plasmid DNA can vary depending on factors such as plasmid size, copy number, and preparation method. Concentrations for plasmid DNA commonly range from 100 ng/µL to several µg/µL.

What are the 4 types of DNA?

There are not four distinct types of DNA, but rather different forms or contexts in which DNA can be found namely Genomic DNA, Mitochondrial DNA, Plasmid DNA, and Complementary DNA.

Why do we measure DNA concentration at 260 nm?

DNA concentration is typically measured at 260 nm because DNA has a strong absorbance of light at this wavelength. The absorption of ultraviolet (UV) light by DNA molecules occurs primarily due to the presence of the aromatic nitrogenous bases, particularly adenine (A) and guanine (G). The absorption of UV light by DNA is based on the principle of the Beer-Lambert law, which states that the absorbance of a sample is directly proportional to the concentration of the absorbing substance and the path length of the light through the sample. The absorbance of DNA at 260 nm is directly related to its concentration.

Web Applications